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Human ESC/iPSC medium
| Cat.No. | ACB17003 | Size | 500ml |
| Name | Human ESC/iPSC medium | Contents | Basal Medium and Supplement |
This product is intended for research use only. This product is not intended to be used for therapeutic or diagnostic uses in humans or animals.
Description: Human ESC/iPSC medium is designed for human induced pluripotent stem cells (iPSCs) and human embryonic stem cells (ESCs). It is serum-free and chemically-defined as all of its ingredients are synthetic chemicals or purified recombinant proteins. Human ESC/iPSC medium supports feeder-free growth and maintenance of human iPSCs or human ESCs on extra-cellular matrix proteins (e.g., BD Matrigel™). Its performance is comparable to the market-leading mTeSR™1 in growth rate, characteristic morphology, pluripotent markers, normal karyotype, and differentiation to germ layers.
Storage and Shelf Life:
| Contents | Size | Storage | Shelf Life |
| Basal Medium | 450ml | 2 to 8°C, protect from light | 6 months |
| 10×Supplement | 50ml | -20°C or below,protect from light | 6 months |
Additional key reagents required or suggested:
- Extracellular matrix proteins, such as Matrigel™ (BD, cat# 354277) or equivalent
- Accutase (Millipore, SCR005) or Collagenase IV (Invitrogen, 17104) or equivalent
- ROCK inhibitor or Thiazovivin or equivalent
Adaptation of growing feeder-free cells to Human ESC/iPSC medium
Human iPSC or ESCs that have been grown in most commonly-used hiPSC/ESC media can be easily adapted into Human ESC/iPSC medium. One round of 1:1 dilution of serum-containing or serum-free medium with Human ESC/iPSC medium is generally sufficient. However, for certain media and cell lines, a longer and moregraduate adaptation may be required.