|Name||Human ESC/iPSC medium||Contents||Basal Medium and Supplement|
This product is intended for research use only. This product is not intended to be used for therapeutic or diagnostic uses in humans or animals.
Description: Human ESC/iPSC medium is designed for human induced pluripotent stem cells (iPSCs) and human embryonic stem cells (ESCs). It is serum-free and chemically-defined as all of its ingredients are synthetic chemicals or purified recombinant proteins. Human ESC/iPSC medium supports feeder-free growth and maintenance of human iPSCs or human ESCs on extra-cellular matrix proteins (e.g., BD Matrigel™). Its performance is comparable to the market-leading mTeSR™1 in growth rate, characteristic morphology, pluripotent markers, normal karyotype, and differentiation to germ layers.
Storage and Shelf Life:
|Basal Medium||450ml||2 to 8°C, protect from light||6 months|
|10×Supplement||50ml||-20°C or below,protect from light||6 months|
Additional key reagents required or suggested:
Adaptation of growing feeder-free cells to Human ESC/iPSC medium
Human iPSC or ESCs that have been grown in most commonly-used hiPSC/ESC media can be easily adapted into Human ESC/iPSC medium. One round of 1:1 dilution of serum-containing or serum-free medium with Human ESC/iPSC medium is generally sufficient. However, for certain media and cell lines, a longer and moregraduate adaptation may be required.